Part:BBa_K4137009:Design

T7+RBS+YebF-GS Linker-chi18h8-6xHis+B1006

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1523
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1036
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 583
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

T7 overexpression, RBS (AAGGAG) efficient protein expression, yebF export chitinase from periplasm to extracellular space via Omp pathway.

Figure 1. Benchling Linear Map View of Chi18H8 construct.

Source

https://parts.igem.org/Part:BBa_K1659003